Part:BBa_K3525001:Design
T7 pro-His-ER sensor-His-T7 ter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 923
Illegal BglII site found at 1420 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1845
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
First, we divided the lacZ coding sequence into two parts, the first 122 amino acids, and the last 123 amino acids. Then, we divided the intein coding sequence into two parts, the first 206 amino acids, and the last 387 amino acids. Most importantly, we combine the sequences in the following order: the first 122 amino acids of lacZ, the first 206 amino acids of the intein, the hera coding sequence, the last 123 amino acids of lacZ, and the last 387 amino acids of the intein.
Source
Rubing Liang, Jing Zhou. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Journal of Biotechnology. Vol.136/S(2008)S1-S772 Jing Zhou, Rubing Liang, Wenhua Wang. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Environmental Science and Technology 4th International Symposium on Novel Carbon Resource Sciences. 2009.